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bwtindex.c 8.29 KB
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Guoqiang QI 提交于 2021-06-11 10:24 . Initial source code
/* The MIT License
Copyright (c) 2008 Genome Research Ltd (GRL).
Permission is hereby granted, free of charge, to any person obtaining
a copy of this software and associated documentation files (the
"Software"), to deal in the Software without restriction, including
without limitation the rights to use, copy, modify, merge, publish,
distribute, sublicense, and/or sell copies of the Software, and to
permit persons to whom the Software is furnished to do so, subject to
the following conditions:
The above copyright notice and this permission notice shall be
included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS
BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN
ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN
CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
*/
/* Contact: Heng Li <lh3@sanger.ac.uk> */
#include <stdio.h>
#include <stdlib.h>
#include <string.h>
#include <unistd.h>
#include <time.h>
#include <zlib.h>
#include "bntseq.h"
#include "bwt.h"
#include "main.h"
#include "utils.h"
#ifdef _DIVBWT
#include "divsufsort.h"
#endif
int is_bwt(ubyte_t *T, int n);
int64_t bwa_seq_len(const char *fn_pac)
{
FILE *fp;
int64_t pac_len;
ubyte_t c;
fp = xopen(fn_pac, "rb");
fseek(fp, -1, SEEK_END);
pac_len = ftell(fp);
fread(&c, 1, 1, fp);
fclose(fp);
return (pac_len - 1) * 4 + (int)c;
}
bwt_t *bwt_pac2bwt(const char *fn_pac, int use_is)
{
bwt_t *bwt;
ubyte_t *buf, *buf2;
int i, pac_size;
FILE *fp;
// initialization
bwt = (bwt_t*)calloc(1, sizeof(bwt_t));
bwt->seq_len = bwa_seq_len(fn_pac);
bwt->bwt_size = (bwt->seq_len + 15) >> 4;
fp = xopen(fn_pac, "rb");
// prepare sequence
pac_size = (bwt->seq_len>>2) + ((bwt->seq_len&3) == 0? 0 : 1);
buf2 = (ubyte_t*)calloc(pac_size, 1);
fread(buf2, 1, pac_size, fp);
fclose(fp);
memset(bwt->L2, 0, 5 * 4);
buf = (ubyte_t*)calloc(bwt->seq_len + 1, 1);
for (i = 0; i < bwt->seq_len; ++i) {
buf[i] = buf2[i>>2] >> ((3 - (i&3)) << 1) & 3;
++bwt->L2[1+buf[i]];
}
for (i = 2; i <= 4; ++i) bwt->L2[i] += bwt->L2[i-1];
free(buf2);
// Burrows-Wheeler Transform
if (use_is) {
bwt->primary = is_bwt(buf, bwt->seq_len);
} else {
#ifdef _DIVBWT
bwt->primary = divbwt(buf, buf, 0, bwt->seq_len);
#else
err_fatal_simple("libdivsufsort is not compiled in.");
#endif
}
bwt->bwt = (u_int32_t*)calloc(bwt->bwt_size, 4);
for (i = 0; i < bwt->seq_len; ++i)
bwt->bwt[i>>4] |= buf[i] << ((15 - (i&15)) << 1);
free(buf);
return bwt;
}
int bwa_pac2bwt(int argc, char *argv[]) // the "pac2bwt" command; IMPORTANT: bwt generated at this step CANNOT be used with BWA. bwtupdate is required!
{
bwt_t *bwt;
int c, use_is = 1;
while ((c = getopt(argc, argv, "d")) >= 0) {
switch (c) {
case 'd': use_is = 0; break;
default: return 1;
}
}
if (optind + 2 > argc) {
fprintf(stderr, "Usage: bwa pac2bwt [-d] <in.pac> <out.bwt>\n");
return 1;
}
bwt = bwt_pac2bwt(argv[optind], use_is);
bwt_dump_bwt(argv[optind+1], bwt);
bwt_destroy(bwt);
return 0;
}
#define bwt_B00(b, k) ((b)->bwt[(k)>>4]>>((~(k)&0xf)<<1)&3)
void bwt_bwtupdate_core(bwt_t *bwt)
{
bwtint_t i, k, c[4], n_occ;
uint32_t *buf;
n_occ = (bwt->seq_len + OCC_INTERVAL - 1) / OCC_INTERVAL + 1;
bwt->bwt_size += n_occ * sizeof(bwtint_t); // the new size
buf = (uint32_t*)calloc(bwt->bwt_size, 4); // will be the new bwt
c[0] = c[1] = c[2] = c[3] = 0;
for (i = k = 0; i < bwt->seq_len; ++i) {
if (i % OCC_INTERVAL == 0) {
memcpy(buf + k, c, sizeof(bwtint_t) * 4);
k += sizeof(bwtint_t); // in fact: sizeof(bwtint_t)=4*(sizeof(bwtint_t)/4)
}
if (i % 16 == 0) buf[k++] = bwt->bwt[i/16]; // 16 == sizeof(uint32_t)/2
++c[bwt_B00(bwt, i)];
}
// the last element
memcpy(buf + k, c, sizeof(bwtint_t) * 4);
xassert(k + sizeof(bwtint_t) == bwt->bwt_size, "inconsistent bwt_size");
// update bwt
free(bwt->bwt); bwt->bwt = buf;
}
int bwa_bwtupdate(int argc, char *argv[]) // the "bwtupdate" command
{
bwt_t *bwt;
if (argc < 2) {
fprintf(stderr, "Usage: bwa bwtupdate <the.bwt>\n");
return 1;
}
bwt = bwt_restore_bwt(argv[1]);
bwt_bwtupdate_core(bwt);
bwt_dump_bwt(argv[1], bwt);
bwt_destroy(bwt);
return 0;
}
int bwa_bwt2sa(int argc, char *argv[]) // the "bwt2sa" command
{
bwt_t *bwt;
int c, sa_intv = 32;
while ((c = getopt(argc, argv, "i:")) >= 0) {
switch (c) {
case 'i': sa_intv = atoi(optarg); break;
default: return 1;
}
}
if (optind + 2 > argc) {
fprintf(stderr, "Usage: bwa bwt2sa [-i %d] <in.bwt> <out.sa>\n", sa_intv);
return 1;
}
bwt = bwt_restore_bwt(argv[optind]);
bwt_cal_sa(bwt, sa_intv);
bwt_dump_sa(argv[optind+1], bwt);
bwt_destroy(bwt);
return 0;
}
int bwa_index(int argc, char *argv[]) // the "index" command
{
extern void bwa_pac_rev_core(const char *fn, const char *fn_rev);
char *prefix = 0, *str, *str2, *str3;
int c, algo_type = 0, is_64 = 0;
clock_t t;
int64_t l_pac;
while ((c = getopt(argc, argv, "6a:p:")) >= 0) {
switch (c) {
case 'a': // if -a is not set, algo_type will be determined later
if (strcmp(optarg, "div") == 0) algo_type = 1;
else if (strcmp(optarg, "bwtsw") == 0) algo_type = 2;
else if (strcmp(optarg, "is") == 0) algo_type = 3;
else err_fatal(__func__, "unknown algorithm: '%s'.", optarg);
break;
case 'p': prefix = strdup(optarg); break;
case '6': is_64 = 1; break;
default: return 1;
}
}
if (optind + 1 > argc) {
fprintf(stderr, "\n");
fprintf(stderr, "Usage: bwa index [-a bwtsw|is] [-c] <in.fasta>\n\n");
fprintf(stderr, "Options: -a STR BWT construction algorithm: bwtsw or is [auto]\n");
fprintf(stderr, " -p STR prefix of the index [same as fasta name]\n");
fprintf(stderr, " -6 index files named as <in.fasta>.64.* instead of <in.fasta>.* \n");
fprintf(stderr, "\n");
fprintf(stderr, "Warning: `-a bwtsw' does not work for short genomes, while `-a is' and\n");
fprintf(stderr, " `-a div' do not work not for long genomes. Please choose `-a'\n");
fprintf(stderr, " according to the length of the genome.\n\n");
return 1;
}
if (prefix == 0) {
prefix = malloc(strlen(argv[optind]) + 4);
strcpy(prefix, argv[optind]);
if (is_64) strcat(prefix, ".64");
}
str = (char*)calloc(strlen(prefix) + 10, 1);
str2 = (char*)calloc(strlen(prefix) + 10, 1);
str3 = (char*)calloc(strlen(prefix) + 10, 1);
{ // nucleotide indexing
gzFile fp = xzopen(argv[optind], "r");
t = clock();
fprintf(stderr, "[bwa_index] Pack FASTA... ");
l_pac = bns_fasta2bntseq(fp, prefix, 0);
fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC);
gzclose(fp);
}
if (algo_type == 0) algo_type = l_pac > 50000000? 2 : 3; // set the algorithm for generating BWT
{
strcpy(str, prefix); strcat(str, ".pac");
strcpy(str2, prefix); strcat(str2, ".bwt");
t = clock();
fprintf(stderr, "[bwa_index] Construct BWT for the packed sequence...\n");
if (algo_type == 2) bwt_bwtgen(str, str2);
else if (algo_type == 1 || algo_type == 3) {
bwt_t *bwt;
bwt = bwt_pac2bwt(str, algo_type == 3);
bwt_dump_bwt(str2, bwt);
bwt_destroy(bwt);
}
fprintf(stderr, "[bwa_index] %.2f seconds elapse.\n", (float)(clock() - t) / CLOCKS_PER_SEC);
}
{
bwt_t *bwt;
strcpy(str, prefix); strcat(str, ".bwt");
t = clock();
fprintf(stderr, "[bwa_index] Update BWT... ");
bwt = bwt_restore_bwt(str);
bwt_bwtupdate_core(bwt);
bwt_dump_bwt(str, bwt);
bwt_destroy(bwt);
fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC);
}
{
gzFile fp = xzopen(argv[optind], "r");
t = clock();
fprintf(stderr, "[bwa_index] Pack forward-only FASTA... ");
l_pac = bns_fasta2bntseq(fp, prefix, 1);
fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC);
gzclose(fp);
}
{
bwt_t *bwt;
strcpy(str, prefix); strcat(str, ".bwt");
strcpy(str3, prefix); strcat(str3, ".sa");
t = clock();
fprintf(stderr, "[bwa_index] Construct SA from BWT and Occ... ");
bwt = bwt_restore_bwt(str);
bwt_cal_sa(bwt, 32);
bwt_dump_sa(str3, bwt);
bwt_destroy(bwt);
fprintf(stderr, "%.2f sec\n", (float)(clock() - t) / CLOCKS_PER_SEC);
}
free(str3); free(str2); free(str); free(prefix);
return 0;
}
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